Figure. Casual mutation in disease resistance genes using KASP_pvr1 and KASP_Bs3 markers in peppers(Holdsworth & Mazourek 2015)

Summary:

• Compared Genotype by Sequencing data from different restriction enzymes to Cucurbita pepo transcript sequences using BLAST

• Developed and tested KASP markers for relevant genes in peppers to make available for breeding work

• Extracted DNA and utilized PCR and DNA sequencing to determine whether a mutation in a predicted gene was responsible for the observed phenotype in peppers.

Relevant Publications

• Wyatt, L. E., Eannetta, N. T., Stellari, G. M., & Mazourek, M. (2012). Development and application of a suite of non-pungency markers for the Pun1 gene in pepper (Capsicum spp.). Molecular Breeding, 30, 1525-1529.

• Holdsworth, W. L., & Mazourek, M. (2015). Development of user-friendly markers for the pvr1 and Bs3 disease resistance genes in pepper. Molecular breeding, 35, 1-5.